PXD056787-1

PXD056787 is an original dataset announced via ProteomeXchange.

Title	Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons
Description	Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons. Lysosomes are membrane-bound organelles critical for maintaining cellular homeostasis. Delivery of biosynthetic lysosomal proteins to lysosomes is crucial to orchestrate proper lysosomal function. However, it remains unknown how the delivery of biosynthetic lysosomal proteins to lysosomes is ensured in neurons, which are highly polarized cells. Here, we developed Protein Origin, Trafficking And Targeting to Organelle Mapping (POTATOMap), by combining trafficking synchronization and proximity-labelling based proteomics, to unravel the trafficking routes and interactome of the biosynthetic lysosomal membrane protein LAMP1 at specified time points.
HostingRepository	PRIDE
AnnounceDate	2025-02-27
AnnouncementXML	Submission_2025-02-27_13:27:53.921.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chun Hei Li
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-14 08:16:24	ID requested
⏵ 1	2025-02-27 13:27:54	announced

10.1038/S41467-024-55052-W;

submitter keyword: Protein synchronization, POTATOMap,Proximity labeling

Ginny G. Farias
contact affiliation	Group leader, Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht 3584 CH, The Netherlands
contact email	g.c.fariasgaldames@uu.nl
lab head
Chun Hei Li
contact affiliation	Utrecht University
contact email	c.h.li@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD056787

PRIDE project URI

• PRIDE
  1. PXD056787
     1. Label: PRIDE project
     2. Name: Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons