PXD056698-1

PXD056698 is an original dataset announced via ProteomeXchange.

Title	The E3 ubiquitin ligase RNF25 protects stalled DNA replication forks
Description	Cancer cells experience high levels of replication stress and have therefore adapted mechanisms to tolerate replication stress. Using a CRISPR screen, we identified RNF25 as a mediator of replication stress tolerance in cancer cells. RNF25-ablation results in ssDNA accumulation, decreased replication fork movement, and increased replication fork degradation dependent on nucleases MRE11 and CtIP. To identify potential interactors of RNF25 in mediating replication stress tolerance, we performed mass-spectrometry analysis of immunopurified RNF25-complexes. We discovered REV7 as a novel binding partner of RNF25. REV7 was previously shown to protect replication forks in a Shieldin-independent but REV1- and REV3L-dependent manner. Further investigation revealed that RNF25 is also epistatic to REV1 and REV3L in a fork protection pathway and that REV7 recruitment to replication forks following stress is dependent on RNF25. Our findings establish a novel role of RNF25 in replication fork protection.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-30_09:39:39.908.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lilly Chiou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-10 11:16:50	ID requested
⏵ 1	2025-06-30 09:39:40	announced

Chiou LF, Jayaprakash D, Droby GN, Zhang X, Yang Y, Mills CA, Webb TS, Barker NK, Wu D, Herring LE, Bowser J, Vaziri C, The RING Finger E3 Ligase RNF25 Protects DNA Replication Forks Independently of its Canonical Roles in Ubiquitin Signaling. bioRxiv, ():(2025) [pubmed]

10.1101/2025.01.09.632184;

submitter keyword: cell culture, IP-MS,Homo sapiens

Cyrus Vaziri
contact affiliation	Departments of Pathology and Laboratory Medicine, Biochemistry and Biophysics, and Lineberger Comprehensive Cancer Center. University of North Carolina at Chapel Hill, United States of America
contact email	cyrus_vaziri@med.unc.edu
lab head
Lilly Chiou
contact affiliation	The University of North Carolina at Chapel Hill
contact email	lfc15@live.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD056698

PRIDE project URI

• PRIDE
  1. PXD056698
     1. Label: PRIDE project
     2. Name: The E3 ubiquitin ligase RNF25 protects stalled DNA replication forks