PXD056626-1

PXD056626 is an original dataset announced via ProteomeXchange.

Title	Molecular mechanism targeting condensin for chromosome condensation
Description	Faithful chromosome segregation requires packaging of the genome on both global and local scales. Condensin plays a crucial role at pericentromeres to resist spindle forces and ensure the oriented attachment of kinetochores to microtubules at mitosis. Here we demonstrate that budding yeast condensin is recruited to pericentromeres through a direct interaction between its Ycg1 subunit and the pericentromeric adaptor protein, shugoshin (Sgo1). We identify a Short Linear Motif (SLiM), termed CR1, within the C-terminal region of Sgo1 which inserts into a conserved pocket on Ycg1. Disruption of this interface abolishes the Sgo1-condensin interaction, prevents condensin recruitment to pericentromeres and results in defective sister kinetochore biorientation in mitosis. Similar motifs to CR1 are found in known and potential condensin binding partners and the Ycg1 binding pocket is broadly conserved, including in mammalian CAP-G proteins. Overall, we uncover the molecular mechanism that targets condensin to define a specialized chromosomal domain.
HostingRepository	PRIDE
AnnounceDate	2024-12-10
AnnouncementXML	Submission_2024-12-10_06:46:24.175.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adele Marston
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 12:50:06	ID requested
⏵ 1	2024-12-10 06:46:24	announced

10.1038/S44318-024-00336-6;

submitter keyword: chromosome segregation, DIA, shugoshin, IPMS, pericentromeres,Condensin

Adele Marston
contact affiliation	The University of Edinburgh
contact email	adele.marston@ed.ac.uk
lab head
Adele Marston
contact affiliation	University of Edinburgh
contact email	adele.marston@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056626
     1. Label: PRIDE project
     2. Name: Molecular mechanism targeting condensin for chromosome condensation