PXD056619-1

PXD056619 is an original dataset announced via ProteomeXchange.

Title	(Phospho-)proteomics analysis of fission yeast cells during mating and cell-cell fusion
Description	In fission yeast Schizosaccharomyces pombe, sexual reproduction is triggered by the absence of nitrogen (N), and relies on crosstalk between multiple signaling pathways, with the inactivation of the TORC1 complex playing a critical role. This process is further elicited by pheromone signaling between the two mating types, P (h+) and M (h-), through GPCR activation of a Ras-MAPK cascade. Once partners are selected, cell projections (shmoos) extend to bring the cells together. Upon pairing, actin fusion foci at the contact site, concentrate hydrolases to digest the cell wall and ensure coordinated fusion. While several kinases and phosphatases are known to promote sexual differentiation and cell fusion, their specific substrates remain largely unknown. To address those questions, we have developed methods to synchronize cell populations during mating and cell-cell fusion and used time course phospho-proteomics to identify dynamic phosphorylation changes. The aim was to uncover the phosphorylation events driving differentiation and morphogenetic changes necessary for cell projection and cell fusion.
HostingRepository	PRIDE
AnnounceDate	2024-12-06
AnnouncementXML	Submission_2024-12-06_09:03:35.565.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophie Martin
SpeciesList	scientific name: Schizosaccharomyces pombe 972h-; NCBI TaxID: 284812;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 08:38:10	ID requested
⏵ 1	2024-12-06 09:03:35	announced

10.1371/JOURNAL.PBIO.3002963;

submitter keyword: sexual differentiation, yeast, nitrogen starvation,Schizosaccharomyces pombe, cell-cell fusion

Martin Sophie
contact affiliation	Department of Molecular and Cellular Biology (MOCEL), University of Geneva, (Previously) Department of Fundamental Microbiology (DMF), University of Lausanne
contact email	sophie.martin@unige.ch
lab head
Sophie Martin
contact affiliation	University of Geneva
contact email	sophie.martin@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD056619

PRIDE project URI

• PRIDE
  1. PXD056619
     1. Label: PRIDE project
     2. Name: (Phospho-)proteomics analysis of fission yeast cells during mating and cell-cell fusion