PXD056608-1

PXD056608 is an original dataset announced via ProteomeXchange.

Title	Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector HopU1
Description	ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana lines expressing the Pseudomonas syringae type III effector HopU1. A non-functional Af1521 G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:18:59.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lennart Wirthmueller
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 05:10:45	ID requested
⏵ 1	2024-10-22 05:19:00	announced

10.1101/2023.09.25.558804;

submitter keyword: Arabidopsis thaliana, type III effector, Pseudomonas syringae, plant pathogen,ADP-ribosylation

Lennart Wirthmueller
contact affiliation	Department Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
contact email	lennart.wirthmueller@ipb-halle.de
lab head
Lennart Wirthmueller
contact affiliation	Leibniz Institute of Plant Biochemistry Dept. Biochemistry of Plant Interactions Weinberg 3 06120 Halle (Saale)
contact email	lennart.wirthmueller@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056608
     1. Label: PRIDE project
     2. Name: Affinity purification of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effector HopU1