PXD056596-2

PXD056596 is an original dataset announced via ProteomeXchange.

Title	Quantitative assessment of a novel device designed for patient-centric sampling of dried plasma using targeted proteomics
Description	The advancements in technology have significantly enhanced proteomics by improving the quality, depth, and timeliness of proteomic data, making mass spectrometry a promising tool for personalized medicine. To enable successful longitudinal population-level monitoring, it is essential to have cost-efficient and convenient sample collection methods that yield reliable results. Microsampling performed by patients in their own homes fulfills this requirement. In this study, we evaluate a novel microsampling device that readily prepares a dried plasma sample from blood collected through a finger prick. The device was assessed quantitatively through a targeted proteomics approach, using a selection of stable isotope-labeled protein fragments as standards for plasma proteins spanning a range from 1,300 µM to 30 nM. All samples were analyzed using selected reaction monitoring to ensure quantitative robustness. The device was assessed both from a storage perspective and across a group of healthy donors to ensure reliable performance between individuals. The dried plasma obtained from the device shows an excellent quantitative correlation with conventional plasma (R > 0.99) and high quantitative precision, with a coefficient of variance (CV) below 10% for 80% of all peptides quantified in the group of healthy donors. All the targets that perform well in the microsampling device also show good long-term stability when stored at room temperature for up to 232 days, further showcasing the potential benefits of collecting samples in a dry format.
HostingRepository	PanoramaPublic
AnnounceDate	2025-07-02
AnnouncementXML	Submission_2025-07-02_20:19:25.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andreas Hober
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 01:13:56	ID requested
1	2025-06-17 09:00:32	announced
⏵ 2	2025-07-02 20:19:26	announced	: Added PubMed ID

Hober A, Henricsson M, Ruckh T, Davidsson P, Challis B, Miliotis T, Quantitative Assessment of a Novel Device Designed for Patient-centric Sampling of Dried Plasma Using Targeted Proteomics. Anal Chem, 97(25):12953-12962(2025) [pubmed]

submitter keyword: microsampling, targeted proteomics, remote sampling, plasma proteomics, srm, selected reaction monitoring, stability

Tasso Miliotis
contact affiliation	Translational Science & Experimental Medicine, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
contact email	tasso.miliotis@astrazeneca.com
lab head
Andreas Hober
contact affiliation	Translational Science & Experimental Medicine, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden
contact email	andreas.hober@astrazeneca.com
dataset submitter

Panorama Public dataset URI