PXD056592-1
PXD056592 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of SpySystem-linked CYP73A5-ATR2 by LC-MSMS |
Description | Eukaryotic cytochrome P450 enzymes, generally colocalizing with their redox partner cytochrome P450 reductase (CPR) on the cytoplasmic surface of organelle membranes, often perform poorly in prokaryotic cells, whether expressed with CPR as a tandem chimera or free-floating individuals, causing a low titer of heterologous chemicals. To improve their biosynthetic performance in Escherichia coli, we here architecturally design self-assembled alternatives of eukaryotic P450 system using reconstructed P450 and CPR, and create a set of N-termini-bridged P450-CPR heterodimers as the counterparts of eukaryotic P450 system with N-terminus-guided colocalization. The covalent counterparts show superior and robust biosynthetic performance; the N-termini-bridged architecture is validated to improve the biosynthesis of both plant and human P450 systems. Furthermore, the architectural configuration of protein assemblies has an inherent effect on the biosynthesis of N-termini-bridged P450-CPR heterodimers. The results suggest that spatial architecture-guided protein assembly could serve as an efficient strategy for improving the biosynthesis of protein complexes, particularly those related to eukaryotic membranes, in prokaryotic and even eukaryotic hosts. |
HostingRepository | iProX |
AnnounceDate | 2024-10-04 |
AnnouncementXML | Submission_2024-10-08_01:02:28.464.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yikui Li |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-10-08 01:01:56 | ID requested | |
⏵ 1 | 2024-10-08 01:02:28 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Eukaryotic P450 system, Spatial organization, Protein architecture, Electron transfer, Biosynthesis, Escherichia coli |
Contact List
Ren Wang | |
---|---|
contact affiliation | Institute of Botany, Jiangsu Province and Chinese Academy of Sciences |
contact email | rwang@cnbg.net |
lab head | |
Yikui Li | |
contact affiliation | Institute of Botany, Jiangsu Province and Chinese Academy of Sciences |
contact email | liyikui@cnbg.net |
dataset submitter |
Full Dataset Link List
iProX dataset URI |