PXD056584-1

PXD056584 is an original dataset announced via ProteomeXchange.

Title	Lymphatic platelet thrombosis limits bone repair by precluding lymphatic transporting DAMPs
Description	In the musculoskeletal system, lymphatic vessels (LVs), which are interdigitated with blood vessels, travel and form an extensive transport network. Blood vessels in bone regulate osteogenesis and hematopoiesis, however, whether LVs in bone affect fracture healing is unclear. Here, we investigated the lymphatic draining function at the tibial fracture sites using near-infrared indocyanine green lymphatic imaging (NIR-ICG) and discovered that lymphatic drainage insufficiency (LDI) started on day one and persisted for up to two weeks following the fracture. Sufficient lymphatic drainage facilitates fracture healing. Furthermore, we identified that lymphatic platelet thrombosis (LPT) blocked the draining lymphoid sinus and LVs, caused LDI, and then inhibited fracture healing, which can be rescued by a pharmacological approach. Moreover, unblocked lymphatic drainage decreased neutrophils and increased M2-like macrophages of the hematoma niche to support osteoblast (OB) survival and bone marrow-derived mesenchymal stem cell (BMSC) proliferation via transporting damage-associated molecular patterns (DAMPs). Lymphatic platelet thrombolysis also benefits senile fracture healing. These findings demonstrate that LPT limits bone regeneration by impeding lymphatic transporting DAMPs. Together, these findings represent a novel way forward in the treatment of bone repair.
HostingRepository	iProX
AnnounceDate	2024-10-05
AnnouncementXML	Submission_2024-10-27_19:49:29.148.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yangkang Zheng
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-08 00:24:12	ID requested
⏵ 1	2024-10-27 19:49:29	announced

Dataset with its publication pending

submitter keyword: Rat thoract duct lymph, fracture, LC-MS

Yongjun Wang
contact affiliation	Shanghai University of Traditional Chinese Medicine
contact email	wangyongjun@shutcm.edu.cn
lab head
Yangkang Zheng
contact affiliation	Shanghai University of Traditional Chinese Medicine
contact email	ethantcheng@126.com
dataset submitter

iProX dataset URI