PXD056531-1

PXD056531 is an original dataset announced via ProteomeXchange.

Title	The proteins identified from Helicobacter pylori strain TN2wt-derived extracellular vesicle (HpEV-TN2wt)
Description	Helicobacter pylori strain TN2wt was suspended in Brucella broth supplemented with 5% depleted exosomes FCS. The initial optical density at 600 nm of the bacterial suspension was adjusted to 0.02 in 200 mL of Brucella broth + 0.5% depleted exosome FCS. H. pylori was grown in a 500 mL flask under microaerophilic conditions, shaking at 100 rpm, at 37oC for 48 h. The H. pylori cells were removed from the culture supernatant by centrifugation at 15,000 x g, for 15 min at 4oC and the bacteria-free supernatant was then filtered through a 0.45 m filter. HpEVs in culture supernatant of H. pylori were harvested by ultracentrifugation at 200,000 x g for 90 min at 4oC using Himac CP80WX Preparative ultracentrifuge (HITACHI, Tokyo, Japan). After removing the supernatant, the HpEVs were washed in 20 mL of 0.9% NaCl solution and collected by ultracentrifugation at 200,000 x g for 90 min at 4oC. HpEVs were resuspended in saline and stored at -80oC. The HpEVs were lysed with 2% sodium dodecyl sulfate/ 7M urea, and equal amounts of proteins from each sample were precipitated with acetone. The proteins were then denatured and reduced with 50% trifluoroethanol and 4 mM dithiothreitol followed by alkylation of the free cysteine residues and trypsinization. The desalted peptides were then separated using liquid chromatography. The mass spectrometer was operated in information-dependent acquisition mode, and acquired spectra were searched against the H. pylori TN2wt protein database.
HostingRepository	jPOST
AnnounceDate	2025-03-17
AnnouncementXML	Submission_2025-03-16_18:59:23.796.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yota Tatara
SpeciesList	scientific name: Helicobacter pylori; NCBI TaxID: 210;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-06 19:22:27	ID requested
⏵ 1	2025-03-16 18:59:24	announced

Subsomwong P, Asano K, Akada J, Matsumoto T, Nakane A, Yamaoka Y, Proteomic Profiling of Extracellular Vesicles Reveals Potential Biomarkers for Helicobacter pylori Infection and Gastric Cancer. Helicobacter, 30(2):e70022(2025) [pubmed]

submitter keyword: Helicobacter pylori, extracellular vesicle

Krisana Asano
lab head
Yota Tatara
contact affiliation	Hirosaki University
dataset submitter

jPOST dataset URI

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