PXD056529-1

PXD056529 is an original dataset announced via ProteomeXchange.

Title	The quantitative proteomic analysis of extracellular vesicles derived from gastric cell line infected with Helicobacter pylori Tx30a, TN2wt strains, and non-infection
Description	Human gastric adenocarcinoma AGS cells were infected with H. pylori Tx30a or TN2wt at a multiplicity of infection = 100 and incubated at 5% CO2,37oC for 48 h. The cells/debris were removed from the cell culture supernatant by centrifugation at 2,000 x g for 30 min at 4oC and filtered through a 0.45 m filter. The EVs were collected by ultracentrifugation at 110,000 x g for 70 min using Himac CP80NX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removing the supernatant, the EVs were washed with PBS and then collected by ultracentrifugation at 100,000 x g for 70 min at 4oC. Finally, the EVs were resuspended in PBS, and stored at -80oC. The AGS-derived EVs were lysed with 2% sodium dodecyl sulfate/ 7M urea, and equal amounts of proteins from each sample were precipitated with acetone. The proteins were then denatured and reduced with 50% trifluoroethanol and 4 mM dithiothreitol followed by alkylation of the free cysteine residues and trypsinization. The desalted peptides were then separated using liquid chromatography. The mass spectrometer was operated in information-dependent acquisition mode, and acquired spectra were searched against the AGS cell protein database. Differential protein expression was analyzed to determine the relative abundances of proteins in EVs derived from AGS cells infected with H. pylori Tx30a or TN2wt, compared to non-infected cells. H. pylori proteins in AGS-derived EV, the spectra were searched against the H. pylori ATCC 51932 (Tx30a) and TN2wt protein database.
HostingRepository	jPOST
AnnounceDate	2025-03-17
AnnouncementXML	Submission_2025-03-16_18:58:35.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yota Tatara
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; phosphorylated L-histidine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-06 18:55:28	ID requested
⏵ 1	2025-03-16 18:58:36	announced

Subsomwong P, Asano K, Akada J, Matsumoto T, Nakane A, Yamaoka Y, Proteomic Profiling of Extracellular Vesicles Reveals Potential Biomarkers for Helicobacter pylori Infection and Gastric Cancer. Helicobacter, 30(2):e70022(2025) [pubmed]

submitter keyword: extracellular vesicle, Helicobacter pylori, gastric cancer

Krisana Asano
lab head
Yota Tatara
contact affiliation	Hirosaki University
dataset submitter

jPOST dataset URI

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