PXD056508-1

PXD056508 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics analysis of WT and tfeb-tfe3s triple knock-out zebrafish adult liver and embryos at 5 days post fertilization
Description	The transcription factors tfeb and tfe3 belong to the MiT/TFE gene family and are known as master regulators of lysosomal function and autophagy. In normal conditions, they are sequestered in the cytosol of the cells and their nuclear translocation is finely regulated in response to a huge array of cell signals; however, the role of these transcription factors during vertebrate development remains unclear. We used zebrafish to investigate tfeb and tfe3 role during embryo development and in adult organs. We generated knock-out (KO) alleles for all the zebrafish tfeb and tfe3s isoforms. Our data show that triple-KO (tfeb-tfe3a-tfe3b) animals are lethal, and these transcription factors are involved in pancreas and liver development. Using the TMT-18plex labeling based quantitative proteomics method and liquid chromatography-tandem mass spectrometry (LC-MS/MS) we performed comparative proteomic analysis of 5 days post fertilization embryos (P1470 files) and adult liver organs (P1408 files) from 2 groups (WT and triple-KO).
HostingRepository	PRIDE
AnnounceDate	2025-05-20
AnnouncementXML	Submission_2025-05-20_09:34:47.412.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056508
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alberto Rissone
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-04 10:34:40	ID requested
⏵ 1	2025-05-20 09:34:47	announced

10.6019/PXD056508;

submitter keyword: zebrafish adult liver, TMT-Multiplex Labeling,zebrafish embryos, LC-MS/MS

Rosa Puertollano
contact affiliation	Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
contact email	puertolr@nhlbi.nih.gov
lab head
Alberto Rissone
contact affiliation	Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD
contact email	alberto.rissone@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056508

PRIDE project URI

• PRIDE
  1. PXD056508
     1. Label: PRIDE project
     2. Name: Quantitative proteomics analysis of WT and tfeb-tfe3s triple knock-out zebrafish adult liver and embryos at 5 days post fertilization