PXD056505-1

PXD056505 is an original dataset announced via ProteomeXchange.

Title	Widespread cancer relevant splicing events driven by the pRb-E2F pathway
Description	E2F is a family of master transcription regulators involved in mediating diverse cell fates, and loss of control of the pathway is regarded as a hallmark of cancer. Recent studies have highlighted the pRb-E2F axis as a regulator of a large cellular network, which in addition to its classical target genes includes RNA splicing and non-coding genes. Here, we have addressed the generality of these effects by studying the role of the other key components of the pathway, including pRb, DP and PRMT5. Like E2F1, both pRb and DP1 influence transcription and alternative RNA splicing (AS) which is additionally impacted by PRMT5 activity. A detailed proteomics analysis of the E2F1 interactome revealed SRSF2 and HNRNPC as partner proteins that assist E2F1 in mediating the effects on RNA splicing. The ability of E2F1 to influence AS activity was apparent as cells progress through the cell cycle and during the DNA damage response. Our results highlight the role of the pRb-E2F pathway in mediating cell cycle regulation of gene expression mediated by transcription and RNA splicing, and provide a mechanistic understanding for how this is likely to occur.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:18:13.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	iolanda Vendrell
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-04 08:27:41	ID requested
⏵ 1	2026-02-08 16:18:17	announced

10.1093/nar/gkag016;

Carr SM, Liu G, Barczak W, Syffudin H, Munro S, Reyna-Jeldes D, Vendrell I, Kessler B, Cribbs AP, Kanapin A, Samsonova A, La Thangue NB, Separate transcription and splicing gene networks are linked and coordinated by the pRb-E2F pathway. Nucleic Acids Res, 54(3):(2026) [pubmed]

submitter keyword: Rb, splicing factor,E2F, alternative splicing, DP

Nicholas B La Thangue
contact affiliation	Laboratory of Cancer Biology, Department of Oncology, University of Oxford Old Road Campus Research Building, Oxford, OX3 7DQ, United Kingdom
contact email	nick.lathangue@oncology.ox.ac.uk
lab head
iolanda Vendrell
contact affiliation	Target Discovery Institute, NDMRB
contact email	iolanda.vendrell@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD056505

PRIDE project URI

• PRIDE
  1. PXD056505
     1. Label: PRIDE project
     2. Name: Widespread cancer relevant splicing events driven by the pRb-E2F pathway