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PXD056502-1

PXD056502 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRed-shifted chlorophyll a absorption in Light-Harvesting Complexes - The case of Trachydiscus minutus
DescriptionPhotosynthetic organisms harvest light for energy. Some eukaryotic algae have specialized in harvesting far-red light by tuning chlorophyll a absorption through a mechanism still to be elucidated. Here, we combined optically detected magnetic resonance and pulsed electron paramagnetic resonance measurements on red-adapted light-harvesting complexes, rVCP, isolated from the freshwater eustigmatophyte alga Trachydiscus minutus to identify the location of the pigments responsible for this remarkable adaptation. The pigments have been found to belong to an excitonic cluster of chlorophylls a at the core of the complex, close to the central carotenoids in L1/L2 sites. A pair of structural features of the Chl a403/a603 binding site, namely the histidine-to-asparagine substitution in the magnesium-ligation residue and the small size of the amino acid at the i-4 position, resulting in a [A/G]xxxN motif, are proposed to be the origin of this trait. Phylogenetic analysis of various eukaryotic red antennae identified several potential LHCs that could share this tuning mechanism.
HostingRepositoryMassIVE
AnnounceDate2024-10-24
AnnouncementXMLSubmission_2024-10-24_06:23:36.360.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeter Konik
SpeciesList scientific name: Trachydiscus minutus;
ModificationListOxidation; Carbamidomethyl
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-04 07:17:06ID requested
12024-10-24 06:23:36announced
Publication List
no publication
Keyword List
submitter keyword: Light-harvesting, Red-shifted LHC, triplet, ODMR, EPR, eustigmatophyte
Contact List
Radek Litvin
contact affiliationCzech Academy of Sciences, Biology Centre, Institute of Plant Molecular Biology, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic; Institute of Chemistry, Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic
contact emailrlitvin@prf.jcu.cz
lab head
Alessandro Agostini
contact affiliationDepartment of Chemical Sciences, University of Padova, via Marzolo 1, 35131, Padova, Italy; Czech Academy of Sciences, Biology Centre, Institute of Plant Molecular Biology, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic
contact emailalessandro.agostini.1@unipd.it
lab head
Peter Konik
contact affiliationUniversity of South Bohemia, Ceske Budejovice
contact emailkonik@prf.jcu.cz
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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