PXD056428-1

PXD056428 is an original dataset announced via ProteomeXchange.

Title	Animal product-free culture conditions for 3D primary human hepatocyte spheroids improve long-term viability and drug metabolism
Description	3D cultures of primary human hepatocytes (3D PHH) are successfully used to reduce and replace the use of animal experiments in biomedical research. Yet, the initial formation of 3D PHH is highly dependent on the supplementation with fetal bovine serum (FBS). However, the molecular composition of FBS and its effects on cultured cells are poorly understood. Moreover, FBS is prone to batch-to-batch variation, immunogenic risk, and lack of adherence to the replacement, refinement and reduction (3Rs) of animal experiments. Here, we demonstrate that FBS can be fully replaced by animal-free substitutes. Specifically, we combined a previously developed animal-free substitute cocktail with a normoglycemic chemically defined culture medium. Morphological and viability evaluations along with global proteomics data, demonstrated that serum-free cultured 3D PHH have equal or superior viability and functional performance of cytochrome P450s, rendering this medium useful for long-term studies and in vitro ADMET applications. This study marks a significant advancement in the development of serum-free culture conditions for primary human hepatocytes, paving the way for more reliable and ethical in vitro studies.
HostingRepository	PRIDE
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-23_02:55:18.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evgeniya Mickols
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-01 08:13:18	ID requested
⏵ 1	2026-01-23 02:55:19	announced

10.1016/j.dmd.2025.100147;

Mickols E, Mohammedamin R, Primpas L, Oredsson S, Karlgren M, Animal product-free formation and cultivation of three-dimensional primary hepatocyte spheroids. Drug Metab Dispos, 53(10):100147(2025) [pubmed]

ProteomeXchange project tag: Liver (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Human, setum-free, FBS, Liver, 3D PHH

Maria Karlgren
contact affiliation	Associate Professor in Molecular Biopharmaceutics, Department of Pharmacy, Uppsala University, Uppsala, Sweden
contact email	maria.karlgren@farmaci.uu.se
lab head
Evgeniya Mickols
contact affiliation	Uppsala University
contact email	evgeniya.mickols@farmaci.uu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD056428

PRIDE project URI

• PRIDE
  1. PXD056428
     1. Label: PRIDE project
     2. Name: Animal product-free culture conditions for 3D primary human hepatocyte spheroids improve long-term viability and drug metabolism