PXD056413-1

PXD056413 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	PEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
Description	In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard sample preparation methods in bottom-up proteomics.
HostingRepository	jPOST
AnnounceDate	2025-01-23
AnnouncementXML	Submission_2025-01-23_00:44:45.826.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naoyuki Sugiyama
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; Acetyl; L-methionine sulfoxide
Instrument	timsTOF Pro 2

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-01 00:12:10	ID requested
⏵ 1	2025-01-23 00:44:46	announced

Publication List

Takemori A, Sugiyama N, Kline JT, Fornelli L, Takemori N, Gel-Based Sample Fractionation with SP3-Purification for Top-Down Proteomics. J Proteome Res, ():(2025) [pubmed]

Takemori A, Sugiyama N, Kline JT, Fornelli L, Takemori N, Gel-Based Sample Fractionation with SP3-Purification for Top-Down Proteomics. J Proteome Res, ():(2025) [pubmed]

Keyword List

submitter keyword: Polyacrylamide gel electrophoresis, Sample fractionation, Top-down proteomics, PEPPI-MS, SP3

submitter keyword: Polyacrylamide gel electrophoresis, Sample fractionation, Top-down proteomics, PEPPI-MS, SP3

Contact List

Nobuaki Takemori
lab head
Naoyuki Sugiyama
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003395/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003395/