PXD056405-1

PXD056405 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the bloodstream forms (slender, stumpy-QS, stumpy-Glyc, intermediate-Glyc) and procyclic forms of Trypanosoma brucei.
Description	In the mammalian bloodstream, proliferative slender forms of T. brucei differentiate into cell cycle-arrested stumpy forms (stumpy-QS) via a quorum sensing mechanism triggered by high parasite density. This transition is thought to protect the host from excessive parasitaemia while preparing the parasites for transmission to the tsetse fly. In this study, we demonstrated that glycerol can also induce the differentiation of slender forms into stumpy forms (stumpy-Glyc), mimicking quorum sensing-induced stumpy forms (stumpy-QS), but with an extended lifespan. Moreover, under tissue-like conditions mimicking the interstitial fluids of adipocytes in the skin (4 mM glucose, 0.25 mM glycerol), glycerol generated proliferative intermediate forms (intermediate-Glyc) capable of differentiating into procyclic forms and establishing infections in tsetse flies. Notably, glycerol also prolonged the survival of quorum-sensing-induced stumpy forms by delaying cell death. Our findings suggest a revised model for T. brucei transmission where quorum sensing controls parasitaemia by producing short-lived stumpy-QS forms, while glycerol secreted by adipocytes in the skin supports the formation of transmissible intermediate-Glyc forms and extends the lifespan of stumpy forms, thus enhancing parasite transmission to tsetse flies.
HostingRepository	PRIDE
AnnounceDate	2026-06-23
AnnouncementXML	Submission_2026-06-23_08:10:30.096.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: NEWT:5691;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-30 13:41:00	ID requested
⏵ 1	2026-06-23 08:10:30	announced

Dataset with its publication pending

submitter keyword: glycerol-induced differentiation, label-free quantitative proteome analysis., bloodstream forms,Trypanosoma brucei, transmission

Frédéric Bringaud
contact affiliation	Univ. Bordeaux, CNRS, MFP, UMR 5234, F-33000 Bordeaux, France
contact email	frederic.bringaud@u-bordeaux.fr
lab head
Dupuy Jean-William
contact affiliation	OncoProt plateform, UAR TBMCore CNRS 3427 INSERM US005 University of Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD056405

PRIDE project URI

• PRIDE
  1. PXD056405
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the bloodstream forms (slender, stumpy-QS, stumpy-Glyc, intermediate-Glyc) and procyclic forms of Trypanosoma brucei.