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PXD056399-1

PXD056399 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructural variation of types IV-A1- and IV-A3-mediated CRISPR interference
DescriptionCRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for gene regulation and plasmid competition. To understand how the type IV-A system associated effector complex achieves this interference, we determined cryo-EM structures of two evolutionarily distinct type IV-A complexes (types IV-38 A1 and IV-A3) bound to cognate DNA-targets in the presence and absence of the type IV-A signature DinG effector helicase. The structures reveal how the effector complexes recognize the protospacer adjacent motif and target-strand DNA to form an R-loop structure. Additionally, we reveal differences between types IV-A1 and IV-A3 in DNA interactions and structural motifs that allow for in trans recruitment of DinG. Our study provides a detailed view of type IV-A mediated DNA-interference and presents a structural foundation for engineering type IV-A-based genome editing tools.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_04:57:12.395.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRimvydė Čepaitė
SpeciesList scientific name: Pseudomonas oleovorans; NCBI TaxID: 301;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-30 09:27:02ID requested
12024-10-08 04:57:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cryo-EM,type IV CRISPR-Cas
Contact List
Patrick Pausch
contact affiliationLSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Saulėtekio al. 7, 10257 Vilnius, Lithuania
contact emailpatrick.pausch@gmc.vu.lt
lab head
Rimvydė Čepaitė
contact affiliationLSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Saulėtekio al. 7, 10257 Vilnius, Lithuania
contact emailrimvyde.cepaite@gmc.vu.lt
dataset submitter
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Dataset FTP location
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