PXD056375-1

PXD056375 is an original dataset announced via ProteomeXchange.

Title	Identification of the S-sulfenylated residues of GSNOR1 in Arabidopsis
Description	The major mechanism by which H2O2 executes its physiological functions is through oxidation of sulfur in the target proteins. Thiol groups of protein cysteine (Cys) could be oxidized by H2O2 to sulfenic, a process also known as S-sulfenylation. Therefore, we reasoned that the activity of GSNOR1 might be regulated by H2O2 through S-sulfenylation. An in vitro S-sulfenylation assay revealed that GSNOR1-His recombinant protein was S-sulfenylated. Because Cys-284 and Cys-285 of GSNOR1 are adjacent and located on the same enzymolysis peptide segment, and also because both of them were identified as S-sulfenylated residues in vivo, to further test whether Cys-284 is S-sulfenylated, Cys-285 was substituted with serine (Ser).
HostingRepository	PRIDE
AnnounceDate	2025-01-23
AnnouncementXML	Submission_2025-01-23_03:50:44.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongyan Guo
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	L-cysteine sulfenic acid; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-30 02:47:29	ID requested
⏵ 1	2025-01-23 03:50:45	announced

10.1016/J.JGG.2025.01.007;

submitter keyword: H2O2

GSNOR1

S-sulfenylation

Jianru Zuo
contact affiliation	State Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
contact email	jrzuo@genetics.ac.cn
lab head
Hongyan Guo
contact affiliation	Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
contact email	hyguo@genetics.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056375
     1. Label: PRIDE project
     2. Name: Identification of the S-sulfenylated residues of GSNOR1 in Arabidopsis