PXD056362-1

PXD056362 is an original dataset announced via ProteomeXchange.

Title	Convergent effects of glucocorticoid stress hormones and amyloid beta in human primary olfactory neuroepithelial
Description	Stressful life events and glucocorticoid (stress) hormones appear to increase risk of Alzheimer’s disease and hasten its progression, but the reasons for this remain unclear. One contributor may be intracellular interactions between amyloid β (Aβ), an early pathological hallmark of Alzheimer’s disease, and glucocorticoids in which 1) glucocorticoids influence Aβ-related cellular pathology and/or Aβ production, and 2) Aβ alters glucocorticoid signaling. To explore both aspects of this possible relationship, the present study investigated whether glucocorticoids and Aβ have overlapping or synergistic effects on the cellular proteome, and whether Aβ influences intracellular glucocorticoid signalling. Olfactory neuroepithelium-derived (ONe) cells from six adult human donors were cultured in vitro and treated with soluble Aβ40 or Aβ42, followed by glucocorticoids (dexamethasone or cortisol). ONe cell treatment with Aβ40 or Aβ42 induced striking proteomic changes in biological processes including innate immunity and mitochondrial function. ONe cells revealed overlapping impacts of Aβ and glucocorticoids, in which- fifteen proteins were significantly altered by both treatments. For twelve of these proteins (seven of which were mitochondrial proteins) dexamethasone counteracted the effects of Aβ. Consistent with these findings, Aβ40 increased, but dexamethasone decreased, ONe cell proliferation. Aβ did not influence intracellular glucocorticoid receptor translocation but there was evidence that some proteins, including complement C3, may be selectively dysregulated by a combination of Aβ and dexamethasone. Overall, this study revealed overlapping effects of glucocorticoids and Aβ, highlighting the potential that glucocorticoid signaling in neurons may counteract some effects of soluble Aβ and exacerbate others inside neurons in ageing and Alzheimer’s disease
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_19:29:55.263.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Wilson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-29 21:12:23	ID requested
⏵ 1	2026-06-28 19:29:56	announced

10.1111/jnc.16263;

Farnum Z, Mani R, Bindoff A, Wilson R, Fiotakis A, Stephens J, Cho E, Mackay-Sim A, Sinclair D, Convergent effects of synthetic glucocorticoid dexamethasone and amyloid beta in human olfactory neurosphere-derived cells. J Neurochem, 169(2):e16263(2025) [pubmed]

submitter keyword: Alzheimer’s disease, glucocorticoid, immune, stress, olfactory neuroepithelium

Dr Richard Wilson
contact affiliation	Proteomics facility, Central Science Laboratory, University of Tasmania
contact email	richard.wilson@utas.edu.au
lab head
Richard Wilson
contact affiliation	University of Tasmania
contact email	richard.wilson@utas.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056362
     1. Label: PRIDE project
     2. Name: Convergent effects of glucocorticoid stress hormones and amyloid beta in human primary olfactory neuroepithelial