PXD056331-1

PXD056331 is an original dataset announced via ProteomeXchange.

Title	Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse
Description	Colorectal cancer molecular signatures derived from omics data can be employed to stratify CRC patients and aid decisions about therapies or evaluate prognostic outcome. However, molecular biomarkers for identification of patients at increased risk of disease relapse are currently lacking. Here, we present a comprehensive multi-omics analysis of a Danish colorectal cancer tumor cohort composed of 412 biopsies from tumors of 371 patients diagnosed at TNM stage II or III. From mass spectrometry-based patient proteome profiles, we classified the tumors into four molecular subtypes, including a mesenchymal-like subtype. As the mesenchymal-rich tumors are known to represent the most invasive and metastatic phenotype, we focused on the protein signature defining this subtype to evaluate their potential as relapse risk markers. Among signature-specific proteins, we followed-up Caveolae-Associated Protein-1 (CAVIN1) and demonstrated its role in tumor progression in a 3D in vitro model of colorectal cancer. Compared to previous omics analyses of CRC, our multi-omics classification provided deeper insights into EMT in cancer cells with stronger correlations with risk of relapse.
HostingRepository	PRIDE
AnnounceDate	2025-03-17
AnnouncementXML	Submission_2025-03-17_12:00:57.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leander Van der Hoeven
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-27 09:08:37	ID requested
⏵ 1	2025-03-17 12:00:58	announced

Dataset with its publication pending

submitter keyword: PhosphoProteomics, Relapse,Colorectal Cancer, Proteomics, Cavin1, LC-MS/MS

Jesper Velgaard Olsen
contact affiliation	Copenhagen University - Novo Nordisk Foundation Center for Protein Research
contact email	jesper.olsen@cpr.ku.dk
lab head
Leander Van der Hoeven
contact affiliation	NNF - CPR
contact email	leander.van.der.hoeven@cpr.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056331
     1. Label: PRIDE project
     2. Name: Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse