PXD056312-1

PXD056312 is an original dataset announced via ProteomeXchange.

Title	Characterization of two urea-utilizing pathways in coral reef ecosystems: implications for the significance of the urea cycle in facing global warming
Description	Urea can serve as nitrogen source for coral holobionts and plays a cruscial role in coral calcification, although the degradation of urea by coral symbionts is not fully understood. In this study, we investigated the urea utilized pathway and the responses of the Symbiodiniaceae family to urea under high temperature conditions. Genome screening revealed that all Symbiodiniaceae species contain the urease (URE) and DUR2 subunit of urea amidolyase (UAD) system. However, only three speciesCladocopium goreaui, Cladopium c92, and Symbiodinium pilosum possess a complete UAD system, including both DUR1 and DUR2. Phylogentic analyses revealed that the UAD system in Symbiodiniaceae clusters more closely with symbiotic bacteria, indicating that horizontal gene transfer of UAD system has occured in coral symbionts. Physiology analysis showed that the symbiodiniacean species Cladocopium goreaui, which containing both URE and UAD, grew better under urea than ammonium conditions, as indicated by higher maximum specific growth rates. Furthermore, most genes of Symbiodiniaceae involved in urea utilization appeared to be stable under various conditions such as heat stress (HS), low light density, and nitrogen deficiency, wheras in ammonium and nitrate transporters were significantly regulated. These relatively stable molecular regulatory properties support sustained urea absorption by Symbiodiniaceae, as evidenced by an increase in δ15N2-urea absorption and the decreases in δ5N-NO3-, and δ15N-NH4+ from cultural environment to Symbiodiniaceae under HS conditions. Token together, this study reveals two distinct urea utilization systems in coral ecosystem and highlights the importance of the urea cycle in coral symbionts when facing fluctuating nitrogen environment in future warming ocean.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:39:04.585.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TC Li
SpeciesList	scientific name: Cladocopium goreaui; NCBI TaxID: 2562237;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; 6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-27 00:10:06	ID requested
⏵ 1	2024-10-08 11:39:05	announced

Dataset with its publication pending

submitter keyword: urea,Symbiodiniaceae, nitrogen environment, urease, coral calcification

Tangcheng Li
contact affiliation	Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, 515063, China
contact email	820195402@qq.com
lab head
TC Li
contact affiliation	.
contact email	820195402@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056312
     1. Label: PRIDE project
     2. Name: Characterization of two urea-utilizing pathways in coral reef ecosystems: implications for the significance of the urea cycle in facing global warming