PXD056260-1

PXD056260 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Role of the sarcin-ricin loop of 23S rRNA in biogenesis of the 50S ribosomal subunit
Description	In this work, we show that 23S rRNA lacking the SRL can be efficiently reconstituted into 50S subunits, which exhibit full peptidyl transferase (PT) activity. Using cryo-EM and proteomic methods, we also further characterize affinity-purified SRL particles isolated from cells. Collectively, these data suggest that the SRL is needed to promote the dissociation of several AFs during 50S subunit biogenesis in the cell.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:20:52.198.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Moon
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480; Bruker Daltonics instrument model

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-25 14:49:06	ID requested
⏵ 1	2025-05-07 03:20:53	announced

Publication List

Aval SF, Seffouh A, Moon KM, Foster LJ, Ortega J, Fredrick K, Role of the sarcin-ricin loop of 23S rRNA in biogenesis of the 50S ribosomal subunit. RNA, 31(4):585-599(2025) [pubmed]
10.1261/rna.080335.124;

Aval SF, Seffouh A, Moon KM, Foster LJ, Ortega J, Fredrick K, Role of the sarcin-ricin loop of 23S rRNA in biogenesis of the 50S ribosomal subunit. RNA, 31(4):585-599(2025) [pubmed]

10.1261/rna.080335.124;

Keyword List

submitter keyword: LC-MSMS, Escherichia coli, 50S ribosomal subunit,Proteomics

submitter keyword: LC-MSMS, Escherichia coli, 50S ribosomal subunit,Proteomics

Contact List

Leonard J. Foster
contact affiliation	Biochemistry and Molecular Biology and Michael Smith Laboratories, University of British Columbia, Canada
contact email	foster@msl.ubc.ca
lab head
Jenny Moon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056260
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056260

PRIDE project URI

Repository Record List

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