PXD056232-1

PXD056232 is an original dataset announced via ProteomeXchange.

Title	Proteomics of TurboID-induced biotinylated proteins from astrocyte and astrocyte endeet after acute peripheral inflammation
Description	The brain vasculature is tightly regulated to control the exchange of substances between the periphery and the brain parenchyma at an interface called the blood-brain barrier (BBB). Astrocytes are glial cells that wrap the brain vasculature with a specialised structure called the endfoot, which is an integral component of the BBB. We generated adeno-associated viruses (AAVs) that express a promiscuous biotin ligase, TurboID, specifically in astrocytes, and developed a method that allows us to define the in vivo protein composition of the astrocyte endfoot with negligible contamination of other cells or other compartments of the astrocyte. Here, we isolated the biotinylated proteins from both the whole astrocyte and astrocyte endfeet from mice with acute peripheral inflammation.
HostingRepository	PRIDE
AnnounceDate	2025-09-11
AnnouncementXML	Submission_2025-09-11_12:10:02.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Blanca Díaz Castro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-25 02:58:16	ID requested
⏵ 1	2025-09-11 12:10:02	announced

Dataset with its publication pending

submitter keyword: mouse, brain, DIA, IP, endfoot, isolation, astrocyte

Blanca Diaz-Castro
contact affiliation	UK Dementia Research Institute University of Edinburgh
contact email	B.Diaz-Castro@ed.ac.uk
lab head
Blanca Díaz Castro
contact affiliation	University of Edinburgh and UK Dementia Research Institute
contact email	b.diaz-castro@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD056232

PRIDE project URI

• PRIDE
  1. PXD056232
     1. Label: PRIDE project
     2. Name: Proteomics of TurboID-induced biotinylated proteins from astrocyte and astrocyte endeet after acute peripheral inflammation