PXD056221-1

PXD056221 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of bone marrow cells from wild-type and Dcaf8 knockout mice
Description	To explore the mechanisms by which DCAF8 deficiency induces functional defects in hematopoietic stem cells with an aging-like phenotype, and given DCAF8’s role as a substrate receptor in the E3 ubiquitin ligase complex, we conducted ubiquitin proteomic analysis on bone marrow cells from wild-type and Dcaf8 knockout mice. This analysis aimed to identify ubiquitinated proteins and assess changes in ubiquitination, providing insights into potential substrates of DCAF8 in murine hematopoietic cells.
HostingRepository	PRIDE
AnnounceDate	2025-10-04
AnnouncementXML	Submission_2025-10-04_08:15:09.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pengfei Xu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-25 00:29:56	ID requested
⏵ 1	2025-10-04 08:15:10	announced

Xu P, Zhang X, Li D, Jiao B, Nie J, Huang Y, Xia Z, Li J, Dan Y, Huang X, Yan L, Zhang R, Huang W, Wang X, Ji S, Cang Y, Ren R, Liu P, Loss of DCAF8 impairs hematopoietic stem cell function with cellular senescence via the DOCK11-CDC42 axis. Blood, 146(12):1462-1475(2025) [pubmed]

10.1182/blood.2024027335;

submitter keyword: Dcaf8

bone marrow

aging

Ping Liu
contact affiliation	Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, Collaborative Innovation Center of Hematology, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China
contact email	liuping@shsmu.edu.cn
lab head
Pengfei Xu
contact affiliation	Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
contact email	pengfxu@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD056221

PRIDE project URI

• PRIDE
  1. PXD056221
     1. Label: PRIDE project
     2. Name: Proteomic analysis of bone marrow cells from wild-type and Dcaf8 knockout mice