PXD056190-1

PXD056190 is an original dataset announced via ProteomeXchange.

Title	TMT-ABPP cysteine profiling of SETDB1 modulators in MCF7 native proteome
Description	We prodiled the cystene engagement of SETDB1 modulators at 10 uM in native MCF7 proteome/lysate with or without spiked of recombinant SETDB1 proteins.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_02:49:23.304.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056190
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ka Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 12:50:20	ID requested
⏵ 1	2025-05-07 02:49:24	announced

10.1038/s41467-025-57005-3;

10.6019/PXD056190;

Uguen M, Shell DJ, Silva M, Deng Y, Li F, Szewczyk MM, Yang K, Zhao Y, Stashko MA, Norris-Drouin JL, Waybright JM, Beldar S, Rectenwald JM, Mordant AL, Webb TS, Herring LE, Arrowsmith CH, Ackloo S, Gygi SP, McGinty RK, Barsyte-Lovejoy D, Liu P, Halabelian L, James LI, Pearce KH, Frye SV, Potent and selective SETDB1 covalent negative allosteric modulator reduces methyltransferase activity in cells. Nat Commun, 16(1):1905(2025) [pubmed]

submitter keyword: ABPP, cysteine,TMT, SETDB1

Ka Yang
contact affiliation	Harvard Medical School
contact email	ka_yang@hms.harvard.edu
lab head
Ka Yang
contact affiliation	Harvard Medical School
contact email	ka_yang@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056190

PRIDE project URI

• PRIDE
  1. PXD056190
     1. Label: PRIDE project
     2. Name: TMT-ABPP cysteine profiling of SETDB1 modulators in MCF7 native proteome