PXD056175-1

PXD056175 is an original dataset announced via ProteomeXchange.

Title	Plasmodium falciparum protein phosphatase PP7 is required for early ring-stage development
Description	We previously reported that the Plasmodium falciparum putative serine/threonine protein phosphatase 7 (PP7) is a high- confidence substrate of the cAMP-dependent protein kinase (PKA). Here we explore the function of PP7 in asexual P. falciparum blood stage parasites. We show that conditional disruption of PP7 leads to a severe growth arrest. We show that PP7 is a calcium-dependent phosphatase that which interacts with calmodulin and calcium-dependent protein kinase 1 (CDPK1), consistent with a role in calcium signalling. Notably, PP7 was found to be dispensable for erythrocyte invasion, but was crucial for ring-stage development, with PP7-null parasites arresting shortly following invasion and showing no transition to ameboid forms. Phosphoproteomic analysis revealed that PP7 may regulate certain PKAc substrates. Its interaction with calmodulin and CDPK1 further emphasiszes a role in calcium signalling, while its impact on early ring development and PKAc substrate phosphorylation underscores its importance in parasite development.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_08:21:12.382.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056175
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 07:52:14	ID requested
⏵ 1	2024-10-08 08:21:12	announced

10.6019/PXD056175;

submitter keyword: Warwick_RTP, apicomplexan parasites, signal transduction, calcium signalling,malaria, serine/threonine phosphatases

Professor David
contact affiliation	Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
contact email	david.baker@lshtm.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056175
     1. Label: PRIDE project
     2. Name: Plasmodium falciparum protein phosphatase PP7 is required for early ring-stage development