PXD056175 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Plasmodium falciparum protein phosphatase PP7 is required for early ring-stage development |
Description | We previously reported that the Plasmodium falciparum putative serine/threonine protein phosphatase 7 (PP7) is a high- confidence substrate of the cAMP-dependent protein kinase (PKA). Here we explore the function of PP7 in asexual P. falciparum blood stage parasites. We show that conditional disruption of PP7 leads to a severe growth arrest. We show that PP7 is a calcium-dependent phosphatase that which interacts with calmodulin and calcium-dependent protein kinase 1 (CDPK1), consistent with a role in calcium signalling. Notably, PP7 was found to be dispensable for erythrocyte invasion, but was crucial for ring-stage development, with PP7-null parasites arresting shortly following invasion and showing no transition to ameboid forms. Phosphoproteomic analysis revealed that PP7 may regulate certain PKAc substrates. Its interaction with calmodulin and CDPK1 further emphasiszes a role in calcium signalling, while its impact on early ring development and PKAc substrate phosphorylation underscores its importance in parasite development. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_08:21:12.382.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD056175 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Andrew Bottrill |
SpeciesList | scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-24 07:52:14 | ID requested | |
⏵ 1 | 2024-10-08 08:21:12 | announced | |
Publication List
Keyword List
submitter keyword: Warwick_RTP, apicomplexan parasites, signal transduction, calcium signalling,malaria, serine/threonine phosphatases |
Contact List
Professor David |
contact affiliation | Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom |
contact email | david.baker@lshtm.ac.uk |
lab head | |
Andrew Bottrill |
contact affiliation | University of Warwick |
contact email | a.r.bottrill@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056175
- Label: PRIDE project
- Name: Plasmodium falciparum protein phosphatase PP7 is required for early ring-stage development