PXD056097-1

PXD056097 is an original dataset announced via ProteomeXchange.

Title	Validation of potential growth-related proteins in chick vitreous during emmetropization using targeted-based proteomics (MRMHR)
Description	The relatively new high-resolution MRM (MRMHR) approach with the Triple-TOF MS running under nanoflow (Which is similar to the SRM/MRM approach) was adopted in this study to solidify further the findings of SWATH-MS results of differentially expressed proteins found across the time points in the chick vitreous for the benefit of low peptide amount consumption. Twelve up-regulated and 26 down-regulated proteins were found across all time points compared to day 7 using SWATH-MS. Several DEPs, such as alpha-fetoprotein, cadherins family group, neurocan, and reelin involved in structural and growth-related pathways, were validated for the first time using MRMHR under this experimental condition.
HostingRepository	PRIDE
AnnounceDate	2024-09-24
AnnouncementXML	Submission_2024-09-23_22:15:11.051.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jimmy Ka-wai Cheung
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-23 08:39:23	ID requested
⏵ 1	2024-09-23 22:15:11	announced

Dataset with its publication pending

submitter keyword: LC-MS, vitreous, MRMHR

Jimmy Ka-Wai
contact affiliation	The Hong Kong Polytechnic univerisity
contact email	kawai.cheung@cevr.hk
lab head
Jimmy Ka-wai Cheung
contact affiliation	Center for eye and vision research, LTD, HK The Hong Kong Polytechinic univerisity, HK
contact email	kawai.cheung@cevr.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056097
     1. Label: PRIDE project
     2. Name: Validation of potential growth-related proteins in chick vitreous during emmetropization using targeted-based proteomics (MRMHR)