PXD056089-1

PXD056089 is an original dataset announced via ProteomeXchange.

Title	Characterization of extracellular micronuclei
Description	Microglia, resident immune cells in the central nervous system, undergo morphological and functional changes in response to signals from the local environment and mature into various homeostatic states. However, niche signals underlying microglial differentiation and maturation remain unknown. Here, we show that neuronal micronuclei transfer to microglia, which is followed by changing microglial characteristics during the postnatal period. Neurons passing through a dense region of the developing neocortex give rise to micronuclei and release them into the extracellular space, before being incorporated into microglia and inducing morphological changes. Two-photon imaging analyses have revealed that microglia incorporating micronuclei tend to slowly retract their processes. Loss of the cGAS gene alleviates effects on micronucleus-dependent morphological changes. Neuronal micronuclei-harboring microglia also exhibit unique transcriptome signatures. These results demonstrate that neuronal micronuclei serve as niche signals that transform microglia, and provide a potential mechanism for regulation of microglial characteristics in the early-postnatal neocortex.
HostingRepository	PRIDE
AnnounceDate	2024-12-29
AnnouncementXML	Submission_2024-12-29_04:19:20.456.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fuminori Tsuruta
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-23 06:15:14	ID requested
⏵ 1	2024-12-29 04:19:20	announced

10.1038/S41593-024-01863-5;

submitter keyword: primary neurons,micronuclei

Fuminori Tsuruta
contact affiliation	University of Tsukuba, Japan
contact email	ftsuruta@gmail.com
lab head
Fuminori Tsuruta
contact affiliation	University of Tsukuba
contact email	ftsuruta@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056089
     1. Label: PRIDE project
     2. Name: Characterization of extracellular micronuclei