PXD056088-1

PXD056088 is an original dataset announced via ProteomeXchange.

Title	Integrated Physical and Proteomic Approaches Dissect the Effect of Leather Degradation by Bacteria
Description	The leather industry has a long history and plays important roles in the global economy. Microbial contamination induced degradation is a common phenomenon in leather. However, the degradation mechanism is not fully understood. In this study, bacteria were isolated from leather artifacts, and how Bacillus licheniformis (Gram-positive bacterium) and Pseudomonas putida (Gram-negative bacterium) corrode cow and sheep leather was explored, respectively. P. putida and B. licheniformis destroyed the morphology of leather and caused obvious color aberration by darkening, greening, and bluing the leather. The tensile strength of sheep leather was significantly damaged by B. licheniformis. Bacteria altered the elemental contents and disrupted the collagen structure of leather to varying degrees. Proteomic analysis found that many proteins were reduced by B. licheniformis in cow and sheep leather, including collagen alpha-1(II) chain, collagen type VI and fibrillar collagen. In contrast, many proteases and peptidases of B. licheniformis were upregulated, such as ATP-dependent Clp protease proteolytic subunit 1, acylaminoacyl-peptidase YuxL, aminopeptidase and carboxypeptidase, suggesting that these enzymes contribute to the degradation of leather proteins. These results highlighted that bacteria cells can effectively degrade leather by secreting proteases and peptidases. This study provided new insights into the conservation and biodegradation of leather and contributed to the green and long-term development of the leather industry.
HostingRepository	PRIDE
AnnounceDate	2025-08-29
AnnouncementXML	Submission_2025-08-28_20:27:57.839.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingyu Lu
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	No PTMs are included in the dataset
Instrument	LC-MS label-free quantitation analysis

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-23 06:01:48	ID requested
⏵ 1	2025-08-28 20:27:58	announced

Lu Q, Jiang N, Cai L, Wang Y, Yang H, Pan J, Wang Y, Zhou Y, Wang H, Integrated Physical and Proteomic Approaches Dissect the Effect of Leather Degradation by Bacteria. J Proteome Res, 24(7):3399-3411(2025) [pubmed]

10.1021/acs.jproteome.5c00090;

submitter keyword: Microorganism

Bacillus licheniformis

Pseudomonas putida

Biodegradation

Leather

Huabing Wang
contact affiliation	Zhejiang University
contact email	wanghb@zju.edu.cn
lab head
Qingyu Lu
contact affiliation	Zhejiang University
contact email	luqingyu1204@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056088
     1. Label: PRIDE project
     2. Name: Integrated Physical and Proteomic Approaches Dissect the Effect of Leather Degradation by Bacteria