PXD056054-1

PXD056054 is an original dataset announced via ProteomeXchange.

Title	Integrative Proteome and Metabolomics Analyses of Cryptococcus neoformans Responses to Melanin Substrates Niger seed and L-DOPA
Description	Melanin, as a pivotal antioxidant pigment, plays a critical role in the pathogenicity of Cryptococcus neoformans. However, the underlying signaling pathways responsible for melanin biosynthesis in C. neoformans are not yet fully elucidated. In this study, proteome and metabolomics analyses were conducted to investigate the response of C. neoformans to melanin substrate Niger seed or L-DOPA. The proteome analysis identified significant differential expression of proteins in cells treated with Niger seed compared to L-DOPA, with distinct functional enrichment patterns observed. Subcellular localization analysis showed unique protein distribution in cells treated with each substrate. Metabolomics analysis revealed distinct metabolic profiles in response to Niger seed or L-DOPA, with notable differences in metabolite regulation between the two treatments. KEGG classification highlighted specific metabolic pathways affected by each substrate. Overall, this study provides valuable insights into the complex regulatory mechanisms underlying C. neoformans response to melanin substrates.
HostingRepository	PRIDE
AnnounceDate	2025-07-23
AnnouncementXML	Submission_2025-07-23_01:43:54.965.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peng Xue
SpeciesList	scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: 235443;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-20 05:09:26	ID requested
⏵ 1	2025-07-23 01:43:55	announced

10.1007/S00284-024-03979-0;

submitter keyword: Cryptococcus neoformans

melanin biosynthesis

substrate-dependent manner

substrate-independent manner

Peng Xue
contact affiliation	Nantong Key Laboratory of Environmental Toxicology, Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong, China
contact email	pengxue@ntu.edu.cn
lab head
Peng Xue
contact affiliation	https://www.ebi.ac.uk/pride/
contact email	pengxue@ntu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD056054

PRIDE project URI

• PRIDE
  1. PXD056054
     1. Label: PRIDE project
     2. Name: Integrative Proteome and Metabolomics Analyses of Cryptococcus neoformans Responses to Melanin Substrates Niger seed and L-DOPA