PXD056040-1

PXD056040 is an original dataset announced via ProteomeXchange.

Title	PRDX6 dictates ferroptosis sensitivity by directing cellular selenium mobilization
Description	Selenium-dependent glutathione peroxidase 4 (GPX4) is the guardian of ferroptosis and prevents unrestrained (phospho)lipid peroxidation by directly reducing phospholipid hydroperoxides (PLOOH) to their corresponding alcohols. However, it remains unclear whether other phospholipid peroxidases can also contribute to ferroptosis prevention, albeit to a varying degree. Here we show that cells lacking GPX4 still exhibit substantial PLOOH reduction capacity, arguing for the presence of alternative PLOOH peroxidases. Mechanistically, we uncover that PRDX6 facilitates intracellular selenium handling, which is crucial for selenium incorporation into selenoproteins, including GPX4.
HostingRepository	PRIDE
AnnounceDate	2025-01-14
AnnouncementXML	Submission_2025-01-14_04:05:13.956.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; monohydroxylated residue; L-cysteic acid (L-cysteine sulfonic acid); selenium containing residue; L-cysteine glutathione disulfide; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-20 00:31:22	ID requested
⏵ 1	2025-01-14 04:05:14	announced

Ito J, Nakamura T, Toyama T, Chen D, Berndt C, Poschmann G, Mour, ã, o ASD, Doll S, Suzuki M, Zhang W, Zheng J, Tr, ü, mbach D, Yamada N, Ono K, Yazaki M, Kawai Y, Arisawa M, Ohsaki Y, Shirakawa H, Wahida A, Proneth B, Saito Y, Nakagawa K, Mishima E, Conrad M, PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization. Mol Cell, 84(23):4629-4644.e9(2024) [pubmed]

10.1016/j.molcel.2024.10.028;

submitter keyword: GPX4, ferroptosis, PRDX6, selenium mobilisation

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Heinrich Heine University Düsseldorf Universitätsstraße 1 40225 Düsseldorf Germany
contact email	gereonl.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056040
     1. Label: PRIDE project
     2. Name: PRDX6 dictates ferroptosis sensitivity by directing cellular selenium mobilization