PXD056025-1

PXD056025 is an original dataset announced via ProteomeXchange.

Title	Microvesicles Derived from Human Bronchial Epithelial Cells Regulate Macrophage Activation During Mycobacterium abscessus Infection
Description	Intercellular communication is important for host immunity in response to bacterial infections. Nontuberculous mycobacterium (NTM), such as Mycobacterium abscessus (M.ab), is a group of environmental bacteria that can cause severe lung infections in individuals with pre-existing lung conditions, including cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). There is limited knowledge understanding the interaction between airway epithelial cells and immune cells during NTM infections. In this study, we characterized microvesicles (MVs) released from M.ab-infected human bronchial epithelial cells and investigated the effect of these MVs on the activation and polarization of THP-1-derived macrophages in cell culture. Our results indicate that MVs released by M.ab-infected human bronchial epithelial cells stimulated the activation of M2-polarized macrophages in cell culture. Additionally, the proteomic analysis for isolated MVs showed that the proteins involved in the cell adhesion pathway were enriched in MVs from M.ab-infected human bronchial epithelial cells compared to the uninfected cells. Among those, the cell surface protein, intercellular adhesion molecule 1 (ICAM-1), regulated the MV internalization by recipient macrophages. In conclusion, our data suggests that in response to M.ab infection, human airway epithelial cells release MVs to modulate the activation of macrophages, which are key cells for mycobacterial intracellular survival in the host.
HostingRepository	PRIDE
AnnounceDate	2026-03-30
AnnouncementXML	Submission_2026-03-29_16:43:06.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Hartson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543); NCBI TaxID: NEWT:561007;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-19 09:17:23	ID requested
⏵ 1	2026-03-29 16:43:07	announced

Guthrie CM, Meeker AC, Self AE, Ramos-Leyva A, Clark OL, Kotey SK, Hartson SD, Liang Y, Liu L, Tan X, Cheng Y, Infection. J Proteome Res, 24(5):2291-2301(2025) [pubmed]

10.1021/acs.jproteome.4c00827;

submitter keyword: Extracellular Vesicles

Microvesicles

Epithelial Cells

Macrophages

Mycobacterium abscessus

Proteomics

Intracellular Communication

Yong Cheng
contact affiliation	Department of Biochemistry and Molecular Biology, Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA
contact email	ycheng@okstate.edu
lab head
Steven Hartson
contact affiliation	Oklahoma State University
contact email	hartson.steve@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056025
     1. Label: PRIDE project
     2. Name: Microvesicles Derived from Human Bronchial Epithelial Cells Regulate Macrophage Activation During Mycobacterium abscessus Infection