PXD055976-1

PXD055976 is an original dataset announced via ProteomeXchange.

Title	AMP-kinase mediates adaptation of glioblastoma cells to conditions of the tumour microenvironment
Description	Proteomic analysis of LNT-299 cells.In this experimental study, we investigated the role of the AMPK-mediated stress response in human GB cells. Mimicking the conditions of the tumour microenvironment we deprived GB cells of glucose and oxygen to characterize metabolic effects and to delineate the relevance of potential downstream targets. AMPK inhibition was modelled by using double-knockout cells of the catalytic subunits α1 and α2. We here report that AMPK activation promotes GB cell survival in the context of both hypoxia and glucose starvation by promoting metabolic adaptation. Furthermore, we found that knockout of the catalytic AMPK subunits resulted in an impaired tumour formation and prolonged survival in vivo. Taken together, the results of this study indicate that the use of AMPK inhibitors could be beneficial to improve GB therapy strategies.
HostingRepository	PRIDE
AnnounceDate	2024-12-02
AnnouncementXML	Submission_2024-12-02_01:54:06.106.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055976
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Münch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-18 06:21:25	ID requested
⏵ 1	2024-12-02 01:54:06	announced

10.6019/PXD055976;

submitter keyword: glioblastoma, TMT,AMPK, LC-MS/MS

Christian Münch
contact affiliation	Director Institute of Molecular Systems Medicine Director Center for Functional Proteomics Goethe University Frankfurt
contact email	ch.muench@em.uni-frankfurt.de
lab head
Christian Münch
contact affiliation	Goethe University Frankfurt
contact email	ch.muench@em.uni-frankfurt.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055976
     1. Label: PRIDE project
     2. Name: AMP-kinase mediates adaptation of glioblastoma cells to conditions of the tumour microenvironment