PXD055970-1

PXD055970 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of porcine seminal extracellular vesicles reveals potential in vivo fertility biomark
Description	Predicting male fertility remains a major challenge in livestock industry. Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that are involved in the regulation of sperm functionality and may therefore be involved in male fertility. The aim of this study was to evaluate whether the protein load of sEVs differs between boars showing differences in fertility when used for artificial insemination (AI). An in-depth quantitative proteomic analysis was performed on two subsets of sEVs of different size (small [S] and large [L]) isolated from boars with different reproductive performance as assessed by farrowing rate (FR) and litter size (LS). Seminal plasma samples from 18 AI-boars (three per boar) from four fertility groups: high FR (H-FR, n=5), low FR (L-FR, n=5), large LS (L-LS, n=4), and small LS (S-LS, n=4) were used. Seminal plasma samples from each fertility group were randomly mixed resulting in three seminal plasma pools per fertility group. Seminal EVs were isolated by size exclusion chromatography and characterized according to MISEV2023 guidelines. Proteomic analysis was performed using a Bruker timsTOF fleX™ instrument with dia PASEF technology. A total of 470 and 726 proteins were quantified in S-sEVs, and 1,801 and 1,834 proteins in L-sEVs from boars with differences in FR and LS, respectively. Differential protein abundance analysis revealed three and 12 proteins quantified (P ≤ 0.05) only in S-sEVs and L-sEVs from L-FR boar samples, and 37 and five proteins quantified (P ≤ 0.05) only in S-sEVs and L-sEVs from L-LS boar samples, respectively. For proteins quantified in all fertility groups, the comparison between L-FR and H-FR boar samples revealed 4 and 40 differentially abundant proteins (DAPs; log2 fold change ≥ ±1, P ≤ 0.05) in S-sEVs and L-sEVs, respectively. When comparing L-LS and S-LS boar samples, 10 and 47 DAPs were identified in S-sEVs and L-sEVs, respectively. These results show that the protein load of sEVs varies between more and less fertile boars and that the DAPs are different between S-sEVs and L-sEVs. These results would support that sEVs proteins would be involved in the fertility of boars and that those that are exclusively and/or differentially abundant between more and less fertile boars could be candidates for fertility biomarkers
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:22:03.751.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF fleX

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-18 04:15:48	ID requested
⏵ 1	2026-02-01 16:22:04	announced

Barranco I, Mart, í, nez-D, í, az P, Parra A, Mart, í, nez-Alborcia MJ, Lucas X, Rodr, í, guez-Mart, í, nez H, Roca J, Proteomic profiling of porcine seminal extracellular vesicles reveals potential in vivo fertility biomarkers. Andrology, 14(2):555-570(2026) [pubmed]

10.1111/andr.70089;

submitter keyword: pig

seminal plasma

extracellular vesicles

Proteomics

diaPASEF

Jordi Roca
contact affiliation	Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain
contact email	roca@um.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055970
     1. Label: PRIDE project
     2. Name: Proteomic profiling of porcine seminal extracellular vesicles reveals potential in vivo fertility biomark