PXD055947-1

PXD055947 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CRL4-DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication
Description	HEK293T cells were transfected with StrepII-FLAG-tagged DCAF12 and, where indicated, treated with the proteasome inhibitor MG-132 for 6 hours. Following treatment, cells were harvested and lysed. Protein complexes associated with StrepII-FLAG-tagged DCAF12 were purified using Strep-TactinXT Superflow resin (IBA Lifesciences, Gottingen, Germany) and eluted with desthiobiotin. Eluted samples were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis at the Proteomics Facility of the Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University (BIOCEV) in Vestec, Czech Republic.
HostingRepository	MassIVE
AnnounceDate	2025-09-01
AnnouncementXML	Submission_2025-09-01_07:53:53.787.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lukas Cermak
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-17 11:57:08	ID requested
⏵ 1	2025-09-01 07:53:54	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: ubiqutin, protein degradation, replication, DatasetType:Proteomics

submitter keyword: ubiqutin, protein degradation, replication, DatasetType:Proteomics

Contact List

Lukas Cermak
contact affiliation	Institute of Molecular Genetics of the ASCR, v. v. i.
contact email	lukas.cermak@img.cas.cz
lab head
Lukas Cermak
contact affiliation	Institute of Molecular Genetics of the ASCR, v. v. i.
contact email	lukas.cermak@img.cas.cz
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097469/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097469/