PXD055917-1

PXD055917 is an original dataset announced via ProteomeXchange.

Title	Selective Labeling of Tyrosine Residues in Proteins: Insights from PTAD Labeling and Tandem Mass Spectrometry Analysis
Description	Designing reagents for protein labeling is crucial for investigating cellular events and developing new therapeutics. Historically, much effort has been focused on labeling lysine and arginine residues due to their abundance on the protein periphery. The chemo-selectivity of these reagents is a challenging yet crucial parameter for deciphering properties specifically associated with the targeted amino acid. Consequently, there is a growing demand for new conjugation reagents and workflows that facilitate selective binding to amino acids other than lysine, cysteine, and arginine. Tyrosine, an aromatic amino acid, occurs moderately on the protein periphery, with its phenolic ring often buried within the tertiary protein structure. This presents a challenging environment for tyrosine-specific protein bioconjugation efforts. The hydrophobic aromatic side chain of tyrosine is known to engage in π-stacking interactions, while the hydroxyl group of the phenyl ring can participate in hydrogen bonding and form tyrosyl radicals involved in electron transfer. 4-phenyl-3H-1,2,4-triazole-3,5(4H)-dione (PTAD) has been previously investigated for its ability to bind to tyrosine. This work presents an extensive structural proteomics investigation of tyrosine labeling across samples of varying complexity, ranging from peptides and proteins to entire cell lysates. Mass spectrometry is utilized to study the behavior of tyrosine-labeled samples through tandem mass spectrometry experiments. We believe these studies will offer valuable insights into tyrosine bioconjugation with PTAD and demonstrate its potential as a covalent labeling reagent for chemical proteomics research.
HostingRepository	PRIDE
AnnounceDate	2024-12-20
AnnouncementXML	Submission_2024-12-20_09:37:30.174.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Saiful Chowdhury
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 14:47:57	ID requested
⏵ 1	2024-12-20 09:37:30	announced

Dataset with its publication pending

submitter keyword: LC-MS, Tyrosine, PTAD

Saiful M Chowdhury
contact affiliation	Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX, 76021
contact email	schowd@uta.edu
lab head
Saiful Chowdhury
contact affiliation	Univeristy of Texas at Arlington
contact email	schowd@uta.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055917
     1. Label: PRIDE project
     2. Name: Selective Labeling of Tyrosine Residues in Proteins: Insights from PTAD Labeling and Tandem Mass Spectrometry Analysis