PXD055914-1

PXD055914 is an original dataset announced via ProteomeXchange.

Title	Protein surface chemistry encodes an adaptive tolerance to desiccation
Description	Cellular desiccation - the loss of nearly all water from the cell - is a recurring stress in an increasing number of ecosystems that can drive proteome-wide protein unfolding and aggregation. For cells to survive this stress, at least some of the proteome must disaggregate and resume function upon rehydration. The molecular determinants that underlie the ability of proteins to do this remain largely unknown. Here, we apply quantitative and structural proteomic mass spectrometry to desiccated and rehydrated yeast extracts to show that some proteins possess an innate capacity to survive extreme water loss. Structural analysis correlates the ability of proteins to tolerate desiccation with their surface chemistry. We find that highly tolerant proteins are responsible for the production of the cell’s building blocks - amino acids, metabolites, and sugars. Conversely, those proteins that are most desiccation-sensitive are involved in ribosome biogenesis and other energy consuming processes. As a result, the rehydrated proteome is preferentially enriched with metabolite and small molecule producers and depleted of some of the cell’s heaviest consumers. We propose this functional bias enables cells to kickstart their metabolism and promote cell survival following desiccation and rehydration.
HostingRepository	PRIDE
AnnounceDate	2025-09-24
AnnouncementXML	Submission_2025-09-23_19:46:08.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haley Moran
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 13:37:47	ID requested
⏵ 1	2025-09-23 19:46:09	announced

10.1016/J.CELS.2025.101407;

submitter keyword: desiccation, proteomics, limited proteolysis, protein structure, yeast, stress response

Shahar Sukenik
contact affiliation	Deparment of Chemistry, Syracuse University
contact email	ssukenik@syr.edu
lab head
Haley Moran
contact affiliation	Johns Hopkins University
contact email	hmoran2@jh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055914
     1. Label: PRIDE project
     2. Name: Protein surface chemistry encodes an adaptive tolerance to desiccation