PXD055912-1

PXD055912 is an original dataset announced via ProteomeXchange.

Title	Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration – PDLIM5 differential interactomics
Description	PDLIM5 is a poorly explored protein, that has been shown to play a role in the maintenance of focal adhesions, likely through its interaction with actin. Our experiments demonstrate that AAK1 phosphorylates PDLIM5 on threonine 290 (T290). The aim of this experiment is to investigate the role of this phosphorylation in PDLIM5’s interaction with focal adhesions. We used co-immunoprecipitation to analyze the interactome of PDLIM5 under physiological conditions. Additionally, we compared its interactome between in wild-type (WT) cells, where it is phosphorylated by AAK1 on T290, and in AAK1 knockout (KO) cells, where it is not. This approach allowed us to compare the PDLIM5 interactome under these two conditions. We discovered that in the absence of AAK1, PDLIM5 interacts more with focal adhesion proteins such as ILK, IQGAP1, and filamins A and B. In the presence of AAK1, PDLIM5 interacts significantly more with various nuclear proteins.
HostingRepository	PRIDE
AnnounceDate	2026-03-20
AnnouncementXML	Submission_2026-03-19_17:25:47.036.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 13:25:41	ID requested
⏵ 1	2026-03-19 17:25:47	announced

Dataset with its publication pending

submitter keyword: LC-MS, DIA, co-immunoprecipitation, PDLIM5, interactomics

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD055912

PRIDE project URI

• PRIDE
  1. PXD055912
     1. Label: PRIDE project
     2. Name: Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration – PDLIM5 differential interactomics