PXD055908-1

PXD055908 is an original dataset announced via ProteomeXchange.

Title	The influence of symbiont identity on the proteomic and metabolomic responses of the model cnidarian Aiptasia to thermal stress
Description	We examined the effect of symbiont identity and heat stress on host metabolome and proteome in the cnidarian-dinoflagellate symbiosis. Exaiptasia diaphana (‘Aiptasia’) was inoculated with its native symbiont (Breviolum minutum) or a non-native symbiont (Symbiodinium microadriaticum; Durusdinium trenchii) and subjected to thermal stress. Host metabolomes were partially distinct at control temperature, however thermal stress caused profiles of anemones containing the two non-native symbionts to become more similar to each other and more distinct from those containing B. minutum. This may reflect symbiont densities; under control conditions, D. trenchii attained 60% and S. microadriaticum 15% of the density attained by B. minutum. At elevated temperature, only D. trenchii-colonised anemones experienced bleaching (60% loss). Combined metabolome and proteome analyses revealed the compounds that make up the thermal response of each symbiosis, either reflecting innate symbiont-specific differences or host-symbiont interactions. We show clear evidence of enhanced nutritional deprivation and cellular stress in hosts harbouring a novel symbiont following temperature stress, independent of the symbiont’s putative thermal resilience. Our findings highlight the physiological limits of partner switching as a means of adaptation to increased temperatures. However, we also offer preliminary evidence suggesting that metabolic functioning of the D. trenchii-Aiptasia association improves over the long-term.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_03:29:20.899.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055908
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Clinton Oakley
SpeciesList	scientific name: Exaiptasia diaphana; NCBI TaxID: 2652724;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 08:50:26	ID requested
⏵ 1	2025-05-07 03:29:21	announced

Lust B, Matthews JL, Oakley CA, Lewis RE, Mendis H, Peng L, Grossman AR, Weis VM, Davy SK, The Influence of Symbiont Identity on the Proteomic and Metabolomic Responses of the Model Cnidarian Aiptasia to Thermal Stress. Environ Microbiol, 27(3):e70073(2025) [pubmed]

10.6019/PXD055908;

10.1111/1462-2920.70073;

submitter keyword: Exaiptasia diaphana, symbiosis, cnidaria, coral bleaching, Symbiodiniaceae

Simon Davy
contact affiliation	School of Biological Sciences, Victoria University of Wellington, New Zealand
contact email	simon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliation	Victoria University of Wellington
contact email	clint.oakley@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055908
     1. Label: PRIDE project
     2. Name: The influence of symbiont identity on the proteomic and metabolomic responses of the model cnidarian Aiptasia to thermal stress