PXD055902-1

PXD055902 is an original dataset announced via ProteomeXchange.

Title	Proteomics of isolated rat manchettes
Description	The manchette is a transient microtubular structure that plays a vital role in nuclear shaping during spermiogenesis. It comprises thousands of microtubules (MTs) that build a scaffold surrounding the distal half of the nucleus. The manchette distributes proteins and vesicles during spermiogenesis in a process called intra-manchette transport (IMT). The current hypothesis is that IMT shares many similarities with intra-flagellar transport (IFT) and utilizes both MTs and filamentous actin (F-actin). However, IMT is still poorly understood as direct visualization of IMT complexes is missing, and the presence of F-actin has not been experimentally shown. Here, we use cryogenic-electron tomography (cryo-ET) to visualize IMT components in manchettes extracted from rat spermatids. Additionaly, we used proteomics to identify and quantify IMT composition. We find that F-actin is an integral part of the manchette with two different spatial organizations, namely bundles and single filaments, indicating two different functional roles. We further uncover that IMT on MTs is mediated by two distinct transport machineries: dynein mediated transport of soluble cargo and dynein independent transport for vesicles. Our results provide new insights into the manchette's function as a transport scaffold, highlighting its significance for the polarization of spermatids during spermiogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-07-18
AnnouncementXML	Submission_2025-07-18_05:24:06.590.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Pérez Pañeda
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 07:54:57	ID requested
⏵ 1	2025-07-18 05:24:07	announced

10.1101/2024.10.16.618660;

submitter keyword: manchette

IMT

microtubules

actin

cell polarization, proteomics, cryo-ET, spermatogenesis, spermiogenesis

Albert J.
contact affiliation	1Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands 2Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact email	a.j.r.heck@uu.nl
lab head
Laura Pérez Pañeda
contact affiliation	Utrecht University
contact email	l.perezpaneda@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD055902

PRIDE project URI

• PRIDE
  1. PXD055902
     1. Label: PRIDE project
     2. Name: Proteomics of isolated rat manchettes