PXD055893-1

PXD055893 is an original dataset announced via ProteomeXchange.

Title	Analysis of the interactome of the RLTPR/CARMIL2 protein in CD4+ T cells
Description	We analyzed the interactome of the mouse protein RLTPR (CARMIL2) using an affinity purification method based on the introduction of a One-Strep-tag (OST) sequence in the C-terminus of the bait protein by genetic engineering of primary mouse T cells. We used mouse models expressing the tagged version of either the wild-type protein or the protein bearing the Q575E mutation. Affinity purification of these OST tagged proteins was performed using Streptactin beads, from T cells either non stimulated, stimulated 2min, 5min or 10min with pervanadate. Each AP-MS purification of an OST- protein is associated with a corresponding control (purification from WT CD4+ T cells) in the same conditions of stimulation. Three replicate biological experiments were performed.
HostingRepository	PRIDE
AnnounceDate	2025-05-12
AnnouncementXML	Submission_2025-05-12_04:13:34.080.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 05:11:33	ID requested
⏵ 1	2025-05-12 04:13:34	announced

Dataset with its publication pending

submitter keyword: AP-MS, primary T cell,RLTPR, interactome, OST tag, CARMIL2

Odile Burlez-Schiltz
contact affiliation	Institute of pharmacology and structural biology, CNRS, Toulouse, France
contact email	gonzalez@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055893
     1. Label: PRIDE project
     2. Name: Analysis of the interactome of the RLTPR/CARMIL2 protein in CD4+ T cells