PXD055890-1

PXD055890 is an original dataset announced via ProteomeXchange.

Title	Laser capture proteomics reveals new candidates for sperm-interacting proteins in the bovine oviduct epithelium
Description	The proximal region of the oviduct (isthmus) serves as a sperm reservoir in many mammalian species. This reservoir is composed of epithelial cells that bind sperm primarily to their apical cilia and ensure the survival of high-quality sperm until ovulation. The species-specific molecular interactions between spermatozoa and the oviduct epithelium, however, are still poorly understood. The aim of this study is to identify new candidates for sperm-interacting proteins in the bovine reservoir using laser capture proteomics. Ipsilateral oviducts were collected from cyclic cows at pre- and post-ovulatory stages. Isthmus and ampulla tissue samples were fixed, paraffin embedded, sectioned and subjected to laser microdissection to establish pools of microsamples separating the apical part of the epithelium, containing potential sperm-interacting proteins, from the basal part, containing proteins not currently located or secreted on the cell surface. Proteins were analyzed by nanoLC-MS/MS, and differentially abundant proteins (DAPs) were identified using t-tests. 505 and 512 proteins could be identified in apical and basal microsamples, respectively. Only 8/141 regional and 1/79 cycle stage-related DAPs were shared between the two epithelium parts, indicating a selective regulation of protein expression. Among the apical proteins, 19 were predicted to be candidates for sperm interactions, including annexins (ANXA) 1, 2, 5 and 8, oviduct glycoprotein 1 (OVGP1), voltage-dependent anion-selective channel proteins (VDAC) 1 and 2, and apolipoprotein A1 (APOA1). This work provides the first proteomic characterization of microdissected cellular compartments of the bovine oviduct epithelium and presents new candidates for improving sperm quality in assisted reproductive technologies.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:55:25.764.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	E Com
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-16 02:54:48	ID requested
⏵ 1	2025-07-27 16:55:26	announced

Mah, é C, Mowinska AM, Albrecht E, Reynaud K, Lavigne R, Com E, Pineau C, Mermillod P, Saint-Dizier M, Schoen J, Laser capture proteomics reveals new candidates for sperm-interacting proteins in the bovine oviduct epithelium. Reproduction, 170(2):(2025) [pubmed]

10.1530/rep-24-0390;

submitter keyword: proteomics,oviduct epithelium, DIA, cell bodies, Data Independant Analysis, apical part, laser microdissection

Charles Pineau
contact affiliation	Protim Core facility - Irset Inserm U1085 - UAR Biosit CNRS 380 Inserm 018
contact email	charles.pineau@inserm.fr
lab head
E Com
contact affiliation	Protim, Irset, Inserm U1085
contact email	emmanuelle.com@univ-rennes1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD055890

PRIDE project URI

• PRIDE
  1. PXD055890
     1. Label: PRIDE project
     2. Name: Laser capture proteomics reveals new candidates for sperm-interacting proteins in the bovine oviduct epithelium