PXD055872-1

PXD055872 is an original dataset announced via ProteomeXchange.

Title	Global proteome characterization of a three-dimensional human chondrocyte cell culture system during chikungunya virus (CHIKV) infection reveals distinct immune and inflammatory signatures
Description	Chikungunya virus (CHIKV) belongs to alphavirus infections which often tend to deliberate musculoskeletal disorders affecting joints, muscles, and bones. Primary cells isolated from patients undergoing total knee replacement were utilized to perceive the effect of virus infection on it, upon isolation and characterization. A well-characterized set of cells was utilized to set the model system in a 2D monolayer and a 3D spheroidal model which was used to evaluate the infection profile of primary chondrocyte cells to CHIKV, the model systems were infected at different multiplicity of infection (MOI) of 0.1, 1 over number of days. Viral titers were determined by plaque assay. We further obtained the global proteome of the infected chondrocytes in its 2D and 3D architecture and analysed various pathways and perturbed proteins in two model systems. Gene expression studies were conducted using total RNA of isolated from cells to study the articular genes upon infection. Our study demonstrates that human chondrocyte cells are permissive to CHIKV infection which is evaluated in both 2D model system and 3D model systems, a comparative study of the proteomes of the chondrocytes was obtained from both 2D and 3D cultures in which distinct protein signatures attributing to cell architecture and immune system were detected.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_16:46:33.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sujatha Sunil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-15 08:35:29	ID requested
⏵ 1	2025-10-05 16:46:34	announced

10.1021/acsomega.5c02832;

Hasan A, Sharma G, Majumder N, Chaudhary S, Mehta D, Vaishya R, Ghosh S, Sunil S, Global Proteome Analysis of a Three-Dimensional Human Chondrocyte Cell Culture System Infected with Chikungunya Virus (CHIKV) Reveals Distinct Immune and Inflammatory Signatures. ACS Omega, 10(37):42480-42493(2025) [pubmed]

submitter keyword: 2-Dimension (2D) monolayer, Chondrocytes,Chikungunya virus (CHIKV), Proteomics, 3-Dimension (3D) spheroid cell culture

Dr. Sujatha Sunil
contact affiliation	Group Leader, Vector Borne Diseases Group, ICGEB, New Delhi, India
contact email	sujatha@icgeb.res.in
lab head
Sujatha Sunil
contact affiliation	International Centre for Genetic Engineering and Biotechnology
contact email	sujatha@icgeb.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055872
     1. Label: PRIDE project
     2. Name: Global proteome characterization of a three-dimensional human chondrocyte cell culture system during chikungunya virus (CHIKV) infection reveals distinct immune and inflammatory signatures