PXD055852-1

PXD055852 is an original dataset announced via ProteomeXchange.

Title	A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.
Description	Pregnancy induces significant physiological changes to support foetal growth, including critical adaptations in pancreatic islets for glucose homeostasis. This study elucidates these adaptations in human islets using immunohistochemistry, with a focus on α- and β-cell metrics, prolactin receptor (PRLR), and serotonin 2B receptor (5-HT2B) expression during pregnancy. Utilising formalin-fixed paraffin-embedded (FFPE) pancreatic tissue sections from pregnant and non-pregnant donors, we employed laser capture microdissection (LCM) to isolate islets followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for proteomic analysis. We observed a 1.9-fold increase in islet area, with α-cell and β-cell areas expanding by 4.3-fold and 1.9-fold, respectively, driven by cell number rather than hypertrophy. Notably, PRLR expression was upregulated in α-cells but not β-cells, and 5-HT2B receptors were absent in β-cells. Proteomic profiling revealed minimal changes in protein expression, suggesting nuanced islet adaptations during pregnancy. Key differentially expressed proteins included cathepsin Z, cyclin-dependent kinase 5, and laminin subunit alpha 4. Our findings highlight the distinct molecular mechanisms of human islets compared to rodent models, underscoring the necessity of human-based studies for advancing understanding of islet adaptations in pregnancy.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-30_02:36:29.393.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon Davis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	timsTOF Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-13 09:19:45	ID requested
⏵ 1	2025-06-30 02:36:29	announced

Dataset with its publication pending

submitter keyword: Human, Diabetes, Pregnancy, Pancreas

Roman Fischer
contact affiliation	Discovery Proteomics Laboratory, University of Oxford, UK
contact email	roman.fischer@ndm.ox.ac.uk
lab head
Simon Davis
contact affiliation	University of Oxford
contact email	simon.davis2@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055852
     1. Label: PRIDE project
     2. Name: A new paradigm of islet adaptations in human pregnancy: insights from immunohistochemistry and proteomics.