PXD055851-1

PXD055851 is an original dataset announced via ProteomeXchange.

Title	Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration - SILAC proteome profiling to determine the impact of acute AAK1/BMP2K inhibition
Description	NAKs (Numb-associated kinases) are a relatively unexplored kinase family that interacts with the endocytic machinery. Among NAK members, AAK1 has been linked to disorders such as neuropathic pain, schizophrenia, and Alzheimer's disease. Our goal is to better understand the cellular pathways regulated by AAK1 and its paralog BMP2K, as their only known role until now has been in endocytosis. Using SILAC labelling for whole cell proteome and phosphoproteome profiling of htert RPE cells pharmacological perturbation of these kinases, we demonstrate that acute inhibition of AAK1 and BMP2K significantly disrupts the abundance and phosphorylation of focal adhesion proteins. This indicates that AAK1 and BMP2K are involved in maintaining focal adhesions in addition to their role in endocytosis.
HostingRepository	PRIDE
AnnounceDate	2026-03-19
AnnouncementXML	Submission_2026-03-19_10:50:41.957.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055851
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-13 08:08:53	ID requested
⏵ 1	2026-03-19 10:50:42	announced

10.6019/PXD055851;

submitter keyword: focal adhesions,LC-MS, SILAC, AAK1, migration, DDA, BMP2K

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD055851

PRIDE project URI

• PRIDE
  1. PXD055851
     1. Label: PRIDE project
     2. Name: Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration - SILAC proteome profiling to determine the impact of acute AAK1/BMP2K inhibition