PXD055844-1

PXD055844 is an original dataset announced via ProteomeXchange.

Title	Regulation of Cortical Neurogenesis by MED13L via Transcriptional Priming and its Implications for MED13L Syndrome
Description	MED13L is highly susceptible to neurodevelopmental disorders, yet the underlying developmental mechanisms remain poorly understood. We developed a gene knockout mouse model of MED13L syndrome to elucidate these mechanisms. Homozygous Med13l-null mice exhibit neonatal lethality and severe cortical neurogenesis deficits due to impaired differentiation of neural progenitors. In contrast, heterozygous Med13l-null mice are viable but display characteristic behavioral deficits of MED13L syndrome, including impaired learning and memory, reduced motor coordination, and heightened anxiety. Heterozygous mice also present with microcephaly and simplified neuronal morphology in the motor cortex. Conditional knockout of Med13l in the forebrain pyramidal lineage corroborated the motor and histological deficits observed. Proteomic, transcriptomic, and single-cell RNA sequencing analyses suggest that MED13L regulates cortical neurogenesis by priming developmental genes for transcriptional activation as a component of the mediator complex. These findings provide insights into the molecular and neurodevelopmental underpinnings of MED13L syndrome, paving the way for potential therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2025-07-09
AnnouncementXML	Submission_2025-07-08_21:26:13.881.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yulan Zhao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-13 03:48:01	ID requested
⏵ 1	2025-07-08 21:26:14	announced

Dataset with its publication pending

submitter keyword: mediator complex, neural progenitors,MED13L syndrome, neurogenesis, cerebral cortex

Xiao-Bing Yuan
contact affiliation	Key Laboratory of Brain Functional Genomics of Shanghai and the Ministry of Education, Institute of Brain Functional Genomics, School of Life Science and the Collaborative Innovation Center for Brain Science, East China Normal University
contact email	xbyuan@brain.ecnu.edu.cn
lab head
Yulan Zhao
contact affiliation	East China Normal University
contact email	ylzhao@imet.ecnu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD055844

PRIDE project URI

• PRIDE
  1. PXD055844
     1. Label: PRIDE project
     2. Name: Regulation of Cortical Neurogenesis by MED13L via Transcriptional Priming and its Implications for MED13L Syndrome