PXD055818-1

PXD055818 is an original dataset announced via ProteomeXchange.

Title	C-terminal amides mark proteins for degradation via SCF/FBXO31 – Expression Proteomics
Description	FBXO31 is a substrate adapter for the SCF-type ubiquitin ligase complex SCF/FBXO31 which ubiquitylates C-terminal amide-bearing proteins (CTAPs), thereby triggering their proteasomal degradation in human cells. FBXO31(D334N) is a dominant de novo mutation found among patients with cerebral palsy. To identify proteins affected by FBXO31(D334N) expression, we engineered HEK293T cells to carry a FBXO31 knockout and rapidly inducible FBXO31 expression using the Shield-1 degron system. WT or D334N variants of FBXO31 were induced for 12h and proteomic changes captured by whole cell proteomics using TMT-based quantification. Deposited files include raw MS spectra, protein-, peptide- and psm-level identification results, and differential gene expression results.
HostingRepository	PRIDE
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2024-11-05_08:49:45.142.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Muhar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	amidated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 06:46:08	ID requested
⏵ 1	2024-11-05 08:49:45	announced

Dataset with its publication pending

submitter keyword: TMT, ubiquitin ligase,FBXO31

Prof. Jacob Corn
contact affiliation	Institute of Molecular Health Sciences, Department of Biology, ETH Zürich; Zürich, Switzerland
contact email	cornj@biol.ethz.ch
lab head
Matthias Muhar
contact affiliation	ETH Zurich
contact email	matthias.muhar@biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD055818

PRIDE project URI

• PRIDE
  1. PXD055818
     1. Label: PRIDE project
     2. Name: C-terminal amides mark proteins for degradation via SCF/FBXO31 – Expression Proteomics