PXD055815-1

PXD055815 is an original dataset announced via ProteomeXchange.

Title	Wolbachia-induced protein changes in D. melanogaster
Description	Wolbachia pipientis is a ubiquitous intracellular bacterium that is known for its manipulation of reproduction in arthropod hosts. Wolbachia has also been shown to colonize virtually all somatic tissues, including the brain, but little is known about the interaction between host and bacterium in these locations. To this end, we studied the effects of Wolbachia infection on the brain of Drosophila melanogaster. Using comparative proteomics, we uncovered the post-translational modification of many proteins within the Drosophila head and body upon infection, with glutamic acid decarboxylase being modified within the head only. Given this enzyme’s role in neurotransmitter synthesis, we next tested how Wolbachia infection impacts various behaviors and GABA production within Drosophila. We discovered an improved response to yeast odors in Wolbachia-infected, mated females compared to their uninfected counterparts. Gross measurements of GABA in whole brains showed no detectable change in GABA abundance upon infection. Treatments with GABA agonist indicated that the behavioral change was not GABA-dependent, leaving the mechanism behind Wolbachia-mediated changes in behavior obscure. Given the multiple protein changes in the Drosophila head upon infection, we propose a model in which Wolbachia drives the modification of glutamic acid decarboxylase, and several metabolic proteins, to increase survival in the specialized niche of the brain. These results give rise to new questions about the Wolbachia-Drosophila relationship and future work will focus on the mechanism through which Wolbachia confers these protein changes.
HostingRepository	PRIDE
AnnounceDate	2025-04-10
AnnouncementXML	Submission_2025-04-10_07:34:02.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Minden
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	amidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 05:59:00	ID requested
⏵ 1	2025-04-10 07:34:02	announced

10.1128/SPECTRUM.02465-24;

submitter keyword: proteomics, brain,Wolbachia, GAD, 2D-DIGE, Drosophila melanogaster

Jonathan Minden
contact affiliation	Carnegie Mellon University
contact email	minden@cmu.edu
lab head
Jonathan Minden
contact affiliation	Carnegie Mellon University
contact email	minden@cmu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD055815

PRIDE project URI

• PRIDE
  1. PXD055815
     1. Label: PRIDE project
     2. Name: Wolbachia-induced protein changes in D. melanogaster