PXD055814-1

PXD055814 is an original dataset announced via ProteomeXchange.

Title	C-terminal amides mark proteins for degradation via SCF/FBXO31 – CTAP discovery
Description	FBXO31 is a substrate adapter for the SCF-type ubiquitin ligase complex SCF/FBXO31 which ubiquitylates C-terminal amide-bearing proteins (CTAPs), thereby triggering their proteasomal degradation in human cells. CTAPs can arise from internal protein cleavage events during which the N-terminal fragment is left with an amidated C-terminus (-0.984016 Da). We searched for such cleavage events (semi-enzymatic C-terminus and C-terminal amidation) in previously published datasets and in own experimental data. These include: - Re-analysis of tissue samples from a deep human proteome atlas (PXD010154) - IP-MS of AARS1, a client of the CTAP reader FBXO31 (MFM-X208) - In vitro fragmentation of purified hemoglobin (MFM-X294, MFM-X298) and tRNA ligase complex (MFM-X314) Experiments are labeled with experiment IDs (MFM-X...). PSM tables contain Experiment IDs and interpretable sample aliases.
HostingRepository	PRIDE
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2024-11-05_08:52:16.110.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Muhar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	amidated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 05:36:18	ID requested
⏵ 1	2024-11-05 08:52:16	announced

Dataset with its publication pending

submitter keyword: C-terminal amide, oxidative stress, ubiquitin ligase,FBXO31

Jacob Corn
contact affiliation	Institute of Molecular Health Sciences, Department of Biology, ETH Zürich; Zürich, Switzerland
contact email	cornj@biol.ethz.ch
lab head
Matthias Muhar
contact affiliation	ETH Zurich
contact email	matthias.muhar@biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD055814

PRIDE project URI

• PRIDE
  1. PXD055814
     1. Label: PRIDE project
     2. Name: C-terminal amides mark proteins for degradation via SCF/FBXO31 – CTAP discovery