PXD055807-1

PXD055807 is an original dataset announced via ProteomeXchange.

Title	Analysis of Lon1 protease traps to identify Arabidopsis protein substrates
Description	The ATP-dependent Lon protease is a key component of protein quality control and highly conserved across the kingdoms of living organisms. In Arabidopsis thaliana Lon1 (At5g26860) is dual targeted to mitochondria and chloroplasts. Lon1 mutant plants (lon1-1) exhibit a post-embryonic growth retardation phenotype accompanied with aberrant mitochondria morphology and reduced respiratory capacity. Even though Lon degrades most of mitochondrial proteins, few substrates of Lon have been identified. To identify the Lon1 substrates, we generated transgenic Arabidopsis lon1-1 mutant plants introducing a proteolytically inactive variant of Lon1 (Lon1trap) targeted to mitochondria by mutating the Serine-Lysine catalytic dyad to Alanine residues. The Lon1trap bears a FLAG-tag at the C-terminus to immunoprecipitate the trap and collect the protein substrates of Lon1 protease immobilized in the proteolytic chamber. A Lon1wt variant that is proteolytically active and carries also a FLAG-tag was introduced to Arabidopsis lon1-1 mutant plants and used as a control. Total proteins were extracted under native conditions from both Lon1trap and Lon1wt transgenic plants of 8 days old seedling, grown vertically on petri dishes at 22oC in the dark from independent biological replicates. The proteins were incubated with anti-FLAG M2-agarose affinity gel to collect the Lon1 traps with the substrates by co-immunoprecipitation.
HostingRepository	PRIDE
AnnounceDate	2025-06-10
AnnouncementXML	Submission_2025-06-10_05:15:58.587.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-12 01:16:12	ID requested
⏵ 1	2025-06-10 05:15:59	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, protein homeostasis, protease, protein quality control,Lon, substrate trapping

Stamatis Rigas
contact affiliation	Department of Biotechnology, Agricultural University of Athens, Iera Odos 75, Athens 118 55, Greece
contact email	srigas@aua.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055807
     1. Label: PRIDE project
     2. Name: Analysis of Lon1 protease traps to identify Arabidopsis protein substrates